Enzyme Engineering
Open Access

Subhash Chand

Publications

• Research Article
  NAD (H) Linked Enzyme Catalyzed Reactions using Coupled Enzymes in a Composite Nanoparticle System
  
  Author(s): Sujata Sinha, Vidya Bhat and Subhash ChandSujata Sinha, Vidya Bhat and Subhash Chand
  
  �Different approaches for cofactor recycling/regeneration have been studied and one of them is having�regenerating enzyme immobilized on some suitable support. Here, we are reporting the cofactor NAD (H) recycling�with the help of free enzymes and enzymes loaded on nanoparticles. Baker?s yeast alcohol dehydrogenase (ADH)�and formate dehydrogenase (FDH) from Candida boidinii were immobilized on alumina nanoparticles and applied�to catalyze the coupled reactions for production of n-propanol. Cofactor regeneration within the reaction cycle was�achieved as a result of collision between enzyme loaded particles and free cofactor. Brownian motion provided�effective interactions among the catalytic components, and thus realized a dynamic shuttling of the cofactor between�the two enzymes to keep the reaction cycles continuing. Maximum recycle rate of 6650 cycles/hr was obtained and it�decreased w.. View More»
  DOI: 10.4172/2329-6674.1000117

  Abstract

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