ISSN: 2476-2059
+44 1478 350008
Sophia Johler
Switzerland
Research Article
Performance of the Assurance GDS® Assay for the Detection of L. monocytogenes in Pure Cultures and Spiked Food Samples
Author(s): Denise Althaus, Claudio Zweifel, Sophia Johler and Roger Stephan
Denise Althaus, Claudio Zweifel, Sophia Johler and Roger Stephan
Listeria monocytogenes is a foodborne pathogen with significant impacts on public health and economy worldwide. Reliable and fast detection of L. monocytogenes is of major importance for both diagnostic laboratories and the food industry. The current study evaluated the performance of the Assurance GDS® assay for the detection of L. monocytogenes in pure cultures and spiked food samples. In the pure culture experiments, the Assurance GDS® assay for Listeria monocytogenes accurately detected the target strains of different serotypes and was correctly negative for a variety of other Listeria species. For reliable detection of L. monocytogenes in pure culture experiments, colony counts >105 cfu/ml were required, which emphasizes the need for an adequate enrichment step. The challenge test experiments (steak tartare, bologna type sausage, Gorgonzola c.. View More»
DOI:
10.4172/2476-2059.1000103