Enzyme Engineering

Enzyme Engineering
Open Access

ISSN: ISSN: 2329-6674

+32 28087017

Shaomin Yan

  • Research Article
    Prediction of Michaelis-Menten Constant in Beta-Cellobiosidases Reaction with Lactoside as Substrate
    Author(s): Shaomin Yan and Guang WuShaomin Yan and Guang Wu

    The Michaelis-Menten constant, Km, is important to understand the characteristics of enzyme and its relationship with substrates and numerous conditions in biochemical reactions. Although the fast development is evidenced in enzymatic research, the Km value in each enzyme under various conditions still needs to be measured individually. On the other hand, the modern computational techniques and bioinformatics provide the opportunity to theoretically predict Km in enzyme with different substrates under various conditions. Cellulose 1,4-beta-cellobiosidase is an enzyme used in cellulose hydrolysis for bio-fuel industry, and huge efforts are made to enhance its efficiency through searching for new strains of beta-cellobiosidase as well as enzymatic engineering. Therefore it is considered important to develop methods to predict the Km value in beta-cellobiosidase?s reaction. In this study, t.. Read More»
    DOI: 10.4172/2329-6674.1000102

    Abstract PDF

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