Medicinal & Aromatic Plants
Open Access
ISSN: 2167-0412
ISSN: 2167-0412
Silosini N
South Africa
Research Article
HPLC Determination of Selected Flavonoid Glycosides and their Corresponding Aglycones in Sutherlandia frutescens Materials
Author(s): Mbamalu ON, Antunes E, Silosini N, Samsodien H and Syce JMbamalu ON, Antunes E, Silosini N, Samsodien H and Syce J
Sutherlandia frutescens is a popular South African plant commercially available in a range of formulations. However, reference standards for quality and stability assessment are lacking. This work reports the development and validation of a reversed phase HPLC method for the analysis of flavonoid glycosides and their corresponding aglycones in S. frutescens products. Five materials containing either leaf powder (LP) or spray-dried aqueous extract (SDAE) of S. frutescens were analysed for flavonoid content. A primary objective was to isolate non-commercially available flavonoid glycoside compounds (sutherlandins) for use as reference standards. Sutherlandins A, B, C and D were successfully isolated, and used, with other flavonoid compounds for HPLC assay development. The developed HPLC method was linear in the range of 0.2 to 60 μg/ml for quercitrin; 0.2 to 120 μg/ml for querceti.. Read More»
DOI:
10.4172/2167-0412.1000246
