Medicinal & Aromatic Plants
Open Access
ISSN: 2167-0412
ISSN: 2167-0412
Petr Cigler
Czech Republic
Research Article
Increased Ginsenosides Production by Elicitation of In vitro Cultivated Panax Ginseng Adventitious Roots
Author(s): Petr Marsik, Lenka Langhansova, Marcela Dvorakova, Petr Cigler, Michal Hruby and Tomas VanekPetr Marsik, Lenka Langhansova, Marcela Dvorakova, Petr Cigler, Michal Hruby and Tomas Vanek
Ginsenosides, which belong to group of triterpenoid saponins, are considered to be main constituents responsible for the biological effects of ginseng drug. Effect of organic and inorganic elicitors and other additives on the ginsenoside biosynthesis and biomass production by the in vitro cultivated adventitious root cultures of Korean ginseng was investigated in present study. Elicitation potential of natural mixtures (coconut water, pineapple extract, casein hydrolyzate, yeast extract, malt extract), organic elicitors (putrescine, spermidine, spermine, jasmonic acid), inorganic ions (calcium) and their complexes with organic acids (titanium ascorbate, titanium citrate) was tested. Changes in concentration of 9 main ginsenosides (Re, Rg1 , Rf, Rg2 , Rb1 , Rb2 , Rc, Rg3 and Rd) were monitored using HPLC-PDA and LC/MS/MS techniques. Jasmonic acid was the most effective elicitor of gins.. View More»
DOI:
10.4172/2167-0412.1000147