Medicinal & Aromatic Plants
Open Access
ISSN: 2167-0412
+44 1300 500008
ISSN: 2167-0412
+44 1300 500008
Mbamalu ON
Discipline of Pharmacology, School of Pharmacy,
Bellville 7535
South Africa
Research Article
HPLC Determination of Selected Flavonoid Glycosides and their Corresponding Aglycones in Sutherlandia frutescens Materials
Author(s): Mbamalu ON, Antunes E, Silosini N, Samsodien H and Syce JMbamalu ON, Antunes E, Silosini N, Samsodien H and Syce J
Sutherlandia frutescens is a popular South African plant commercially available in a range of formulations. However, reference standards for quality and stability assessment are lacking. This work reports the development and validation of a reversed phase HPLC method for the analysis of flavonoid glycosides and their corresponding aglycones in S. frutescens products. Five materials containing either leaf powder (LP) or spray-dried aqueous extract (SDAE) of S. frutescens were analysed for flavonoid content. A primary objective was to isolate non-commercially available flavonoid glycoside compounds (sutherlandins) for use as reference standards. Sutherlandins A, B, C and D were successfully isolated, and used, with other flavonoid compounds for HPLC assay development. The developed HPLC method was linear in the range of 0.2 to 60 μg/ml for quercitrin; 0.2 to 120 μg/ml for querceti.. View More»
DOI:
10.4172/2167-0412.1000246