Medicinal & Aromatic Plants
Open Access
ISSN: 2167-0412
+44 1300 500008
ISSN: 2167-0412
+44 1300 500008
Fabian M Dayrit
Katipunan Ave., Loyola Heights, Quezon City, 1008
Philippines
Research Article
Untargeted Bioassay Strategy for Medicinal Plants: In Vitro Antidiabetic Activity and 13C NMR Profiling of Extracts from Vitex negundo L
Author(s): Myrnille Joy B Zabala, Lolita G Lagurin and Fabian M DayritMyrnille Joy B Zabala, Lolita G Lagurin and Fabian M Dayrit
Bioassay-guided fractionation is the principal method for the identification of active constituents in medicinal plants. By design, this method aims to identify the most active compound in a complex mixture with the objective of discovering novel drug candidates. Described here is a complementary method for the identification of known bioactive compounds in medicinal plants which is untargeted and which takes advantage of the large NMR database of known natural products and availability of statistical software. This untargeted bioassay strategy is demonstrated as a proof of principle in the determination of the antidiabetic compounds in Vitex negundo L. Crude methanol and ethanol extracts, and chloroform, ethyl acetate and aqueous fractions of V. negundo L. were prepared and tested for their in vitro antidiabetic potential using the glucose diffusion retardation assay and the in vitro.. View More»
DOI:
10.4172/2167-0412.1000313