Medicinal & Aromatic Plants
Open Access
ISSN: 2167-0412
+44 1300 500008
ISSN: 2167-0412
+44 1300 500008
Corey D Broeckling
Colorado State University
Others, Proteomics and Metabolomics Facility,
Fort Collins, CO 80523
United States
Research Article
Rice Bran Extracts Inhibit Invasion and Intracellular Replication ofSalmonella typhimurium in Mouse and Porcine Intestinal Epithelial Cells
Author(s): Irfan A Ghazi, Job O Mapesa, Jessie R Wilburn, Jan E Leach, Sangeeta Rao, Corey D Broeckling, Anna McClung, Elizabeth P Ryan* and Iman ZareiIrfan A Ghazi, Job O Mapesa, Jessie R Wilburn, Jan E Leach, Sangeeta Rao, Corey D Broeckling, Anna McClung, Elizabeth P Ryan* and Iman Zarei
Dietary rice bran supplementation has been shown to inhibit Salmonella fecal shedding in animals. The aim of this study was to determine if bran extracts from two distinct rice varieties, Lijiangxintuanheigu (LTH)d and Sanhuangzhan-2 (SHZ-2), differentially inhibit Salmonella enterica serovar Typhimurium invasion and intracellular replication. Rice bran extracts were tested in vitro using mouse small intestine epithelial (MSIE) and intestinal porcine epithelial cells (IPEC-J2). Fluorescent labeled Salmonella was detected using fluorescence microscopy and culture based methods. Non-targeted metabolomics using ultra-performance liquid chromatography- mass spectrometry (UPLC-MS) was performed on LTH and SHZ-2 rice bran extracts. LTH bran extract dose-dependently reduced entry and intracellular replicat.. View More»
DOI:
10.4172/2167-0412.1000271