Advancements in Genetic Engineering
Open Access
ISSN: 2169-0111
ISSN: 2169-0111
Arvind Chhabra
Farmington, Connecticut
Tanzania
Editorial
Advances in Genome Engineering Approaches
Author(s): Arvind ChhabraArvind Chhabra
Targeted genome editing is essential for functional characterization of a gene of interest. Targeted gene inactivation via homologous recombination made it feasible to create gene knockout animal models to ascertain the physiological role of the target genes; however, lower efficiency of site specific insertion of the genetically modified construct through homologous recombination has limited a wider applicability of this approach. Development of targeted gene knockdown through RNA interferce (RNAi) offered a cost effective, high-throughput alternative to homologous recombination, however, RNAi-mediated gene knockdown is incomplete, produces experiment to experiment variation, and could provide only a temporary inhibition of the gene function. Development of genome engineering methodologies utilizing nucleases linked to the guide sequences targeting a gene of interest, such as Zinc Fi.. View More»
DOI:
10.4172/2169-0111.1000e107