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Project utilizes Massively Parallel Signature Sequencing (MPSS) to profile RNA populations from a large number of rigorously collected mouse tissues. MPSS utilizes microbeads to capture and quantify tags for mRNAs isolated from tissues or cells; identity of the mRNAs is determined by sequencing of cDNA fragments attached to the beads. The C57BL/6J mouse was chosen as the source for tissues since the mouse offers consistency of genetic background, premorbid state, and tissue acquisition, as well as detailed genome information. These are essential elements for the creation of a meaningful gene expression dataset.
Related Journals of Mouse Transcriptome
Transcriptomics, Human Genetics & Embryology, Cloning & Transgenesis, Journal of Molecular and Genetic Medicine, Incorporative Mouse Genome, Advances in Genetics, American Journal of Medical Genetics, Animal Genetics