Medicinal & Aromatic Plants
Open Access
ISSN: 2167-0412
+44 1300 500008
ISSN: 2167-0412
+44 1300 500008
Anand Singh Bisht
College of Horticulture,
Bharsar, Pauri, Uttarakhand
India
Research Article
In-vitro Mutagenesis Induction to Improve Abiotic Stress in Tissue Cultured Plantlet of Picrohiza kurroa Royle ex. Benth: An Endangered Plant of Western Himalayas, India
Author(s): Surjeet Singh Bisht, Anand Singh Bisht and Rajendar Singh ChauhanSurjeet Singh Bisht, Anand Singh Bisht and Rajendar Singh Chauhan
The study is focused to establish an improved salt and drought tolerant Picrohiza kurroa Royle ex Benth, an endangered medicinal plant of Western Himalayas through mutagenesis system in conjunction with in- vitro regeneration technique. Regeneration using leaf as explants from mature plant of characterized germplasm is beneficial because the source plant is not damaged. Efficient callus formation i.e., 85% was achieved in modified MS medium at 2,4-D at 3.5 mg L-1. Ethyl methane sulfonate (EMS) a chemical mutagen was used to induce mutation in the callus biomass under in-vitro condition. Relatively decreases in callus biomass were observed as the dose of mutagenic chemical increased from 0.0 to 0.8 mM. Selection of mutants callus lines were also investigated against salt (NaCl) and drought (mannitol) tolerance level by using its various concentrations i.e., 50 to 250 mM L-1. In mutagen.. View More»
DOI:
10.4172/2167-0412.1000287