Mycobacterial Diseases
Open Access
ISSN: 2161-1068
ISSN: 2161-1068
Tomoko Suzuki
Japan
Research Article
Use of Ultrasonication as a Rapid Pretreatment Method for MALDI-TOF MS of Mycobacterial Samples
Author(s): Katsunao Niitsuma, Shizuko Koshiba, Miwako Saitou, Tomoko Suzuki, Kinuyo Chikamatsu, Akiko Takagi and Satoshi Mitarai
Katsunao Niitsuma, Shizuko Koshiba, Miwako Saitou, Tomoko Suzuki, Kinuyo Chikamatsu, Akiko Takagi and Satoshi Mitarai
Background: In Japan, matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) is used as a simple and accurate method for mycobacterial identification, but existing pretreatment techniques are time-consuming and laborious.
Objective: We characterized a new pretreatment technique that extracts proteins using ultrasonic disruption.
Methods: We compared this new technique with the current pretreatment method and examined the usefulness of ultrasonication, including its use in mycobacterial inactivation. A total of 174 mycobacterial isolates were tested, including 50 Mycobacterium tuberculosis complex strains, 57 Mycobacterium avium strains, 55 Mycobacterium intracellulare strains, and 12 Mycobacterium kansasii strains. The ultrasonic pretreatment method.. View More»
DOI:
10.4172/2161-1068.1000260