Mycobacterial Diseases
Open Access

Soniya Sharma

Publications

• Research Article
  Detection of Mutations in rpob Gene of Clinically Isolated M. Tuberculosis by DNA Sequencing
  Author(s): Soniya Sharma and Molly Madan Soniya Sharma and Molly Madan
  
  Aim: A population-based study was performed to detect the mutations in 81-bp rifampicin resistance determining region (RRDR) of rpoB gene in clinically isolated M. tuberculosis strains. Methods: Polymerase chain reaction (PCR) mediated direct DNA sequencing was used for rapid detection of rifampicin resistance of M. tuberculosis. Results: Among the 150 isolates, 115 were rifampicin sensitive and exhibited a wild-type pattern on PCR mediated direct sequencing, and remaining 35 isolates were found to be resistant. The codons most frequently involved in mutation were codon 531(40%), 526(23%), and 516(15%).Total twenty four kinds of mutation, which 18 point mutation, 4 insertion & 2 deletion were observed in 81-bp RRDR region of rpoB gene. Conclusion: The sequencing analysis for genotypic.. View More»
  DOI: 10.4172/2161-1068.1000156

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