Transcriptomics: Open Access
Open Access

Singh DK

Publications

• Research Article
  Genetic Diversity Cucumber Using Inter Simple Sequence Repeats (ISSR)
  Author(s): Singh DK, Rajani Tewari, Singh NK and Shashank S SinghSingh DK, Rajani Tewari, Singh NK and Shashank S Singh
  
  Cucumber is an important vegetable crop grown worldwide. Genetic diversity and similarity among 11 cucumber genotypes viz. PCUCP-2, PCUCP-3, Nun-3139, Nun-3121, Nun-3141, Infinity, Isatis and Kian and PCUC-8, PCUC- 15 and PCUC-28 were grown under greenhouse conditions in G. B. Pant University of Agriculture and Technology, Pantnagar. Fresh and young leaves were collected from each genotype. The leaves were cut into small bits and ground to powder using liquid nitrogen. Genomic DNA was then isolated using modified CTAB method. The genetic diversity and similarity was estimated by using 8 ISSR markers. The 6 ISSR primers generated a total of 49 polymorphic alleles. On the basis of ISSR data, dendrogram clustered the genotypes into two major clusters and five sub-clusters. Among all the genotypes a total of 40-100% polymorphism was observed. The number of alleles produced by different pr.. View More»
  DOI: 10.4172/2329-8936.1000129

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