Mycobacterial Diseases
Open Access
ISSN: 2161-1068
+44 1478 350008
ISSN: 2161-1068
+44 1478 350008
Shumaila N. M. Hanif
Faculty of Medicine,
P.O.BOX 24923 13110, Safat
Kuwait
Research Article
The Natural Expression of Genes Encoding Major Antigens of Rd1 and Rd9 in M. Tuberculosis and other Mycobacteria
Author(s): Shumaila N. M. Hanif, Rajaa Al-Attiyah and Abu Salim MustafaShumaila N. M. Hanif, Rajaa Al-Attiyah and Abu Salim Mustafa
The natural expression of genes of five major antigenic proteins present in the genomic region of difference (RD)1, i.e. pe35, ppeE68, esxA and esxB , and RD9, i.e. esxV , was determined, at mRNA and protein levels, in M. tuberculosis, M. bovis BCG, and environmental mycobacterial species of M.avium and M.vaccae . The expression of mRNA was determined using total mycobacterial RNA and gene-specific forward and reverse primers in reverse transcription-polymerase chain reaction (RT-PCR), and the expression of proteins was determined by Western immunoblot analysis of mycobacterial sonicates with anti-sera containing protein-specific antibodies raised in rabbits. Furthermore, the expression of each protein in various sub-cellular fractions of M.tuberculosis was studied by Western immunoblot analysis of cell walls, culture filtrates and sonicates of M... View More»
DOI:
10.4172/2161-1068.1000105