Mycobacterial Diseases
Open Access
ISSN: 2161-1068
+44 1478 350008
ISSN: 2161-1068
+44 1478 350008
Selvaraj R
India
Research Article
Comparison of Variants of Carbol Fuchsin & Phenol in Ziehl Neelsen Staining to Detect AFB
Author(s): Jaya Chandra T, Selvaraj R and Sharma YVJaya Chandra T, Selvaraj R and Sharma YV
Introduction: Sputum smear microscopy is the mainstay in the rapid diagnosis of pulmonary tuberculosis. Ziehl Neelsen staining is popularly used smear microscopy techniques.
Method: We evaluated the use of 0.1% Basic Fuchsin, 5% phenol containing primary staining reagent (Solution ‘A’) and 0.1% Basic Fuchsin, 7.5% phenol (Solution ‘B’) in the diagnosis of pulmonary tuberculosis. Results are compared with standard.
Results: Out of 196 sputum samples smear positivity were 9.7%, 7.6% and 9.2% respectively with standard
method and Solution ‘A’ and Solution ‘B’.
Conclusion: Even though with some reduction in expenditure, reagents concentrations should not be altered... View More»
DOI:
10.4172/2161-1068.1000131