Mycobacterial Diseases
Open Access
ISSN: 2161-1068
+44 1478 350008
ISSN: 2161-1068
+44 1478 350008
Prasanta Kumar Das
Intermediate Reference Laboratory,
State TB Demonstration cum training Centre Kolkata-10 West Bengal
India
Research Article
Mitigating PCR /Amplicon Contamination in a High Risk High Burden Mycobacterial Reference Laboratory in a Resource Limited Setting
Author(s): Prasanta Kumar Das, Somtirtha B Ganguly and Bodhisatya MandalPrasanta Kumar Das, Somtirtha B Ganguly and Bodhisatya Mandal
Background: Nucleic acid amplification techniques have become important machineries in the diagnosis of several diseases in clinical laboratories. PCR contamination/Amplicon Contamination leading to false positivity remains a major concern in these laboratories. Prevention of these contaminations in establishing these Molecular Biology Laboratories has been very crucial over the years. Though closed system PCRs has substantial reduction in the PCR contamination rates the conventional probe based hybridization methods continues to show occurrence of contamination for various reasons.
The Study involved checking the crucial parameters as well as the probable candidates of causing the contamination at a high burden setting. Bringing out the most effective interventions in controlling PCR contaminations for future endeavors stood as a priority. The study explore.. View More»
DOI:
10.4172/2161-1068.1000261