Fungal Genomics & Biology
Open Access
ISSN: 2165-8056
ISSN: 2165-8056
Otilia Macarisin
Tanzania
Research Article
Dominant Selectable Markers for Penicillium spp. Transformation and Gene Function Studies
Author(s): Wayne M Jurick II, Hui Peng, Verneta L Gaskins, Ivana Vico, Jiujiang Yu, Otilia Macarisin, Wojciech J Janisiewicz and Kari A PeterWayne M Jurick II, Hui Peng, Verneta L Gaskins, Ivana Vico, Jiujiang Yu, Otilia Macarisin, Wojciech J Janisiewicz and Kari A Peter
Penicillium spp. has been genetically manipulated and gene function studies have utilized single gene deletion strains for phenotypic analysis. Fungal transformation experiments have relied on hygromycin and hygromycin phosphotransferase (hph) as the main dominant selectable marker (DSM) system in Penicillium spp. This poses a limitation on the number of loci that can be analyzed and complemented in reverse genetic studies. Additionally, many economically important Penicillium spp. have not been evaluated to determine the utility of additional chemicals that can serve as DSMs. Therefore, six compounds were examined for 15 blue mold strains and their Minimum Inhibitory Concentrations (MICs) determined. Phleomycin, neomycin and G418 were deemed ineffective, as Penicillium spp. growth was observed on media amended with 1000 μg/ml of each compound. The effica.. View More»
DOI:
10.4172/2165-8056.1000153