Advances in dairy Research
Open Access
ISSN: 2329-888X
+44 1300 500008
ISSN: 2329-888X
+44 1300 500008
Nermin Sajic
Netherlands
Research Article
ELISA for Raw and Heat-Treated Cows and Buffalos Milk in the Milk of Other Species and Sources
Author(s): Willem Haasnoot, Nermin Sajic, Karen Doorn Essers, Lucia Streppel and Ron VerheijenWillem Haasnoot, Nermin Sajic, Karen Doorn Essers, Lucia Streppel and Ron Verheijen
Due to the lower prices of cow’s and buffalo’s milk, fraudulent mixing with higher priced milk of other species and sources is economically attractive but illegal and dangerous for allergic consumers. For the rapid detection of cow’s milk, several immunoassays are available but most of them lack the possibility to detect heat-treated cow’s milk due to denaturation of the target protein(s). In the present indirect enzyme-linked immunosorbent assay (ELISA), a mouse monoclonal antibody (Mab) raised against the bovine milk protein ?-casein is labelled with the enzyme horseradish peroxidase and the binding to the bovine ?-casein-coated wells of a 96-wells microtiter plate is inhibited by free bovine ?-casein in the sample or standard. The Mab recognises a 5 amino acids-containing epitope on the glycomacropeptide (GMP) part of bovine ?-casein which is absent in ?-cas.. View More»
DOI:
10.4172/2329-888X.1000118