Mycobacterial Diseases
Open Access
ISSN: 2161-1068
+44 1478 350008
ISSN: 2161-1068
+44 1478 350008
Mohammad Javad Nasiri
NRITLD,
Tehran, 19556, P.O: 19575/154
West Indies
Research Article
Evaluation of Molecular Diversity of Mycobacterium tuberculosis Strains By Polymorphisms in RD Regions
Author(s): Ehsan Sharifipour, Mohammad Javad Nasiri, Parissa Farnia, Mohadeseh Mozafari, Shiva Irani and Ali Akbar VelayatiEhsan Sharifipour, Mohammad Javad Nasiri, Parissa Farnia, Mohadeseh Mozafari, Shiva Irani and Ali Akbar Velayati
Background: The region of differences (RDs) polymorphisms is a potential molecular epidemiology method to distinguish origins of Mycobacterium tuberculosis (MTB). Till date, 68 RDs were identified in MTB. This study was designed to investigate highly polymorphic regions (RD1, RD150 and RD181) among MTB strains isolates from Iranian Pulmonary tuberculosis patients (PTB).
Method and materials: A total of the 190 MTB isolates were identified by conventional and molecular methods. Thereafter, Spoligotyping and RD typing (RD1, RD150 and RD181) were applied to genotype these strains.
Results: The most frequent spoligotype belong to Haarlem (n=72, 37.8%) followed by CAS (n=37, 19.4%), T1 (n=18, 9.4%) and Beijing (n=11, 5.7%) lineages. Deletion in RD181 was only identified among Beijing lineage.
Conclusion: .. View More»
DOI:
10.4172/2161-1068.1000153