Mycobacterial Diseases
Open Access
ISSN: 2161-1068
+44 1478 350008
ISSN: 2161-1068
+44 1478 350008
M. Natrajan
India
Research Article
Expression and Analysis of CXCL8 and CXCL10 Chemokines in Human Skin
Lesions Infected with M.leprae
Author(s): Krishan Dutta Rawat, Mamta Chahar, PVJ Reddy, Nalini Srivastava, U.D.Gupta, M. Natrajan, V.M. Katoch, Kiran Katoch and D.S. ChauhanKrishan Dutta Rawat, Mamta Chahar, PVJ Reddy, Nalini Srivastava, U.D.Gupta, M. Natrajan, V.M. Katoch, Kiran Katoch and D.S. Chauhan
This study is focused to analyze the expression profile of CXCL8 and CXCL10 chemokines genes and to address the contribution of these chemokines in late phase of M.leprae infection in human skin lesion samples. In this study skin biopsy samples from leprosy patients (n=35) were collected including BL-LL=12, BB=14, BT=8 and healthy volunteers (n=3). All the biopsy samples were collected in RNALater for mRNA expression study as well as 10% buffered formalin (BF) for histopathologic analysis. Total RNA was isolated from collected samples and cDNA was prepared. Level of mRNA expression of CXCL8 and CXCL10 chemokine genes was measured via q-PCR and insitu RT-PCR to locate the presence of CXCL8 and CXCL10 chemokines inside M. leprae infected tissue. The mRNA expression of CXCL8 was found relatively elevated in lepromatous (BL-LL) skin sampl.. View More»
DOI:
10.4172/2161-1068.1000208