Mycobacterial Diseases
Open Access
ISSN: 2161-1068
ISSN: 2161-1068
Leila de Souza Fonseca
Brazil
Research Article
Cloning, Expression and Characterization of Fusion Proteins Based on Peptides of Rv1980c Disrupting Rv3019c Sequence and Evaluation of its Potential Immunoreactivity in Pulmonary Tuberculosis Sera
Author(s): Isabela Gama Sardella, Ana Carla de Paulo Mulinari, Leila de Souza Fonseca and Maria Helena Féres SaadIsabela Gama Sardella, Ana Carla de Paulo Mulinari, Leila de Souza Fonseca and Maria Helena Féres Saad
Mycobacterium tuberculosis-specific antigens (Ag) would be of important value in developing immunodiagnostic test for tuberculosis (TB), however human heterogeneous recognition of Ag epitopes may result in specificity variation of the test. In this work, we described the construction of two fusion proteins, based on two peptides from MPT-64 disrupting MT-10 Ag, F1-MT10.3 (1M-40S):CE15 (173G-187A):MT10.3 (41S-96) and F2- MT10.3 (1M-40S):MPT64 (91L-205A):MT10.3 (41S-96), and their potential immunoreactivity in TB sera. These fusion genes were cloned in expression vector, inserted in E. coli, and their proteins were expressed and purified. Using ELISA technique purified fused proteins and single full antigens were evaluated for IgA, IgM and IgG in sera from individuals diagnosed with pulmonary tuberculosis (TB) and controls with other pulmonary disease. The F1 construction generated a ne.. View More»
DOI:
10.4172/2161-1068.1000183