Fungal Genomics & Biology
Open Access
ISSN: 2165-8056
ISSN: 2165-8056
Jay D Evans
BARC-East Building 306, Beltsville Maryland 20705
Tanzania
Research Article
Effective Silencing of Dicer Decreases Spore Load of the Honey Bee Parasite Nosema ceranae
Author(s): Qiang Huang, Yanping Chen, Peter Neumann, Wenfeng Li and Jay D EvansQiang Huang, Yanping Chen, Peter Neumann, Wenfeng Li and Jay D Evans
Nosema ceranae is an intracellular parasite that infects honey bee mid-gut epithelial cells. Previously, we identified microRNA-like small RNAs and found evidence for expression of the N. ceranae gene coding Dicer. The Dicer protein is critical for small regulatory RNA synthesis and RNA interference. In order to test the effects of Dicer on N. ceranae reproduction, we designed small interfering RNAs (siRNAs) to suppress Dicer gene expression. After inoculating honey bees with N. ceranae spores, siRNAs targeting the gene for Dicer were fed to individual honey bees for three days post infection at 24 h intervals (siRNA-Dicer group). Additional honey bees were fed with non-specific siRNAs (siRNA-scramble group). The third group of honey bees was inoculated with N. ceranae spores without any siRNA treatment (infection group). Overall, siRNA-Dicer significantly inhibited the spore producti.. View More»
DOI:
10.4172/2165-8056.1000144