Fungal Genomics & Biology
Open Access

Han AB Wösten

Publications

• Research Article
  Transcriptome Analysis of Zones of Colonies of the ΔflbA Strain of Aspergillus niger
  Author(s): Pauline Krijgsheld and Han AB WöstenPauline Krijgsheld and Han AB Wösten
  
  Wild-type colonies of Aspergillus niger grow and secrete enzymes at their periphery when they are grown on an agar medium. Inactivation of the sporulation gene flbA results in colonies that not only secrete proteins at their periphery, but also in central zones. This is accompanied by a more complex secretome, growth throughout the mycelium, and by thinner cell walls. Here, gene expression was studied at the periphery, an intermediate zone, and the centre of wild-type and ?flbA colonies using whole genome microarrays. Heterogeneity in gene expression was not reduced in ?flbA colonies when compared to wild-type colonies, despite decreased heterogeneity in zonal secretion, sporulation and growth. It was shown that 1152 genes had a fold change difference in expression ? 2, when the averaged expression profiles of the zones of the wild-type were compared with those of ?flbA colonies. This ge.. View More»
  DOI: 10.4172/2165-8056.1000109

  Abstract PDF

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