Mycobacterial Diseases
Open Access
ISSN: 2161-1068
+44 1478 350008
ISSN: 2161-1068
+44 1478 350008
Gerd Pluschke
Swiss Tropical and Public Health Institute,
Switzerland
Research Article
Localization of Mycobacterial Antigens by Immunofluorescence Staining of Agarose Embedded Cells
Author(s): Andrea Vettiger, Nicole Scherr, Marie-Thérèse Ruf, Katharina Röltgen and Gerd PluschkeAndrea Vettiger, Nicole Scherr, Marie-Thérèse Ruf, Katharina Röltgen and Gerd Pluschke
Here we report the adaptation of an immunofluorescence staining method for mycobacterial antigens. Our approach is based on agarose embedding followed by thin sectioning of the bacterial samples. We demonstrate that this technique has great potential for protein localization studies, without the use of fluorescence tagged fusion proteins, which is of special interest for slow growing mycobacterial species... View More»
DOI:
10.4172/2161-1068.1000150