Journal of Nutrition & Food Sciences
Open Access
ISSN: 2155-9600
ISSN: 2155-9600
Department of Nutrition and Food Technology, University of Chile, Santiago, Chile
Research Article
Effects of Chia Mucilage on Intestinal Glucose Absorption: An In vitro Model in Intestinal Epithelial Cells
Author(s): Alexandra Valenzuelas, Monica Andrews, Matias Rivera and Miguel Arredondo*
Soluble fiber intake is essential in maintaining Glucose (Glc) homeostasis. However, the evidence attributes this effect only to viscous soluble fibers. Within the highly viscous and little explored fibers, is the Chia Mucilage (ChM), a seed present for centuries in our continent. The most accepted mechanism of action currently for viscous fibers is that they delay gastric emptying and intestinal transit, leading to better Glc management, but few studies have evaluated their action at the level of the intestinal barrier and the cells of this epithelium. Our objective was to evaluate the effect of ChM on Glc dialyzability and relative expression of SGLT-1 and GLUT-2. We performed in vitro static digestion assays for glucose and we measured Glc dialyzability and amylase activity, in the presence of ChM. Then, in Caco-2 cells challenged with Glc (25 and 35 mM) and ChM (1 mg/mL) .. View More»