Journal of Nutrition & Food Sciences
Open Access
ISSN: 2155-9600
ISSN: 2155-9600
Center Unidad de Investigación Biomolecular en Cardiología, Hospital de Cardiología, CMN-SXXI, IMSS, Ciudad de México, Mexico
Research Article
Nutritional Recovery and its Effect on Tryptophan-5-Hydroxylases Expression, Cell Number and on Changes Caused by Intrauterine Growth Restriction in the Developing Brain
Author(s): Gabriel Manjarrez-Gutiérrez*, Jorge Hernández-Rodríguez and José Antonio Mondragón-Herrera
Objective: The aim of this work was to determine whether the changes in tryptophan-5-hydroxylase (TPH) 1 or 2 brain expression caused by intrauterine growth restriction (IUGR) are maintained or return to normal in the nutritionally-recovered undernourished offspring (UNDO) of rats.
Methods: The experimental procedure was: Wistar female rats were subjected to a protein-caloric-restricted diet during gestation. At birth, part of the newborn UNDO pups were fed by their own mothers (UNDO Group), and others were fed by normal control mothers to allow their nutritional recovery (NR Group); pups from normal control dams were fed by them (C Group). On days 1, 15 and 21, brainstem tissue, at the level of dorsal raphe nucleus (DRN), was obtained, in order to determine L-tryptophan (L-Trp), serotonin (5-HT) and TPH activity. In addition, TPH 1 or 2 expre.. View More»
DOI:
10.35248/2155-9600.20.10.1000774