Transcriptomics: Open Access
Open Access

Wright WR

Publications

• Research Article
  The Role of TLR2 in Cigarette Smoke-Induced Gene Induction
  Author(s): The Role of TLR2 in Cigarette Smoke-Induced Gene InductionThe Role of TLR2 in Cigarette Smoke-Induced Gene Induction
  
  Background: Previous work form our group has shown that innate immune cells perceive cigarette smoke as an inflammatory and xenobiotic stimulus, which alters the immune response to invading pathogens. It is unclear as to the specific cellular and molecular mechanisms driving cigarette smoke-induced inflammation, but we have demonstrated that this response is, in part, driven by Toll-Like Receptor (TLR) 2 in innate immune cells in vitro and in mice in vivo . Methodology/Principle findings: To address the impact TLR2/6, has on cigarette smoke-induced gene induction, HEK293 cells were permanently transfected with TLR2/6 or Null vector, and were stimulated with 10% cigarette smoke extract (CSE) for 8 h. Total RNA was extracted and the transcriptome analysed using Illumina Bead Chip arrays. In HEK293 Null cells, CSE induced 33 genes and down-regulated 41. In HEK293 TLR2/6 cells, CSE induce.. View More»
  DOI: 10.4172/2329-8936.1000102

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