Translational Medicine
Open Access
ISSN: 2161-1025
+44 1223 790975
ISSN: 2161-1025
+44 1223 790975
Tanvir S. Khatlani
Tanzania
Research Article
Clinical and Prognostic Significance of R282W p53 Gene Mutation in North India Patients with Non Small Cell Lung Cancer
Author(s): Jamsheed Javid, M.Masroor, AB Rashid Mir, Imtiyaz Ahamad, Shazia Farooq, Prasant Yadav, Mariyam Zuberi, Sameer Goru, Sheikh shanawaz, P.C Ray, Anant Mohan, Ajaz Ah Bhat, Tanvir S. Khatlani and Alpana SaxenaJamsheed Javid, M.Masroor, AB Rashid Mir, Imtiyaz Ahamad, Shazia Farooq, Prasant Yadav, Mariyam Zuberi, Sameer Goru, Sheikh shanawaz, P.C Ray, Anant Mohan, Ajaz Ah Bhat, Tanvir S. Khatlani and Alpana Saxena
Background: p53 plays a central role in protecting the integrity of the genome. Its activity is ubiquitously lost in cancers, either by inactivation of its protein (p53 pathway) or by mutation in the p53 gene, thereby indicating its importance in understanding cancer and as a therapeutic target. Given the high frequency of the hotspot R282W p53 gene mutation in our NSCLC patients, we have evaluated the association of R282W mutation with the progression of the malignancy. Methods: Blood DNA was extracted from cases. The R282W hotspot p53 gene mutation was detected by using ASO-PCR. Most of the NSCLC patient’s submitted samples for EGFR gene mutation analysis. Results: The clinical significance of R282W hotspot p53 gene mutation in exon 8, codon 268 (C>T) was studied in hundred clinically confirmed NSCLC patients samples. Sixty two of h.. View More»
DOI:
10.4172/2161-1025.1000110