Translational Medicine

Translational Medicine
Open Access

ISSN: 2161-1025

Qiang Shi

Qiang Shi

IR Iran

  • Research Article
    Hippocampal Neuron Protecting Effect of Propofol Against Hypoxia/Reoxygenation via Inducing Nerve Growth Factor
    Author(s): Ya-Ni Feng, Qiang Shi, Hong Ma and Jun FangYa-Ni Feng, Qiang Shi, Hong Ma and Jun Fang

    Propofol, an intravenous anesthetic agent, exhibits neuroprotective effects against cerebral ischemia–reperfusion injury. Here, we used a model of Hypoxia/Re-Oxygenation (H/R) injury to examine the hippocampal neuroprotective effect of propofol, and explored the role of Nerve Growth Factor (NGF) and NGF receptor TrkA in this action. Rat hippocampal neuron cells were subjected to H/R with different concentrations propofol, the viability and apoptosis of the cells were then determined by MTT assay and annexin V flow cytometry, respectively. Meanwhile, expression of NGF and TrkA were measured by RT-PCR and Western blot. The results showed that H/R significantly reduced viability and increased apoptosis of cultured hippocampal neuron cells, along with the significantly decreased expressions of NGF and TrkA. However, pretreatment of propofol recovered the expressions of NGF and the r.. View More»
    DOI: 10.4172/2161-1025.1000123

    Abstract PDF

Relevant Topics