Journal of Chromatography & Separation Techniques
Open Access
ISSN: 2157-7064
+44 1300 500008
ISSN: 2157-7064
+44 1300 500008
Attarde DL
Mahatma Gandhi Vidyamandir’s Pharmacy College,
Panchavati, Nashik, Maharashtra
India
Research Article
Premnine HCl Estimation in Selected Formulations of ‘Dashmul’ and in
Chloroform Extract of Premna integrifolia L. by a Selective, Validated and
Developed HPTLC Fingerprint Method
Author(s): Attarde DL, Pal SC and Bhambar RSAttarde DL, Pal SC and Bhambar RS
HPTLC technique developed as validated method for estimation of Premnine HCl in P. integrifolia chloroform extract (PI-AK) and in selected marketed formulations ‘Dashmul arishta’,‘Dashmul kadha’ in 3 × 3 batches as per ICH guidelines. Premnine HCl (Pr-s) was isolated as per literature from P. integrifolia and focused first time as standard bioactive marker for quantification. Developed mobile phase Toluene: Acetone: Diethylamine (7:2:1) for Pr-S gave Rf 0.59 at λ max 283 nm in densitometric scan, focused for specificity, fingerprint and estimation study in PI-AK and selected formulation successively. Linearity assessed in range of 8 to 16 μg /band with regression coefficient of 0.9983, LOD 0.742 μg/Band, LOQ 2.225, also robust for Pr-S. Accuracy for % recovery performed on extract as well on formulation, further subjected for precision study .. View More»
DOI:
10.4172/2157-7064.1000352