Immuno Affinity Chromatography Journals action could be a methodology of separating organic chemistry mixtures supported a extremely specific interaction like that between substance and protein, accelerator and substrate, or receptor and substance. The stationary part is often a gel matrix, usually of agarose; a linear sugar molecule derived from alga. Typically the place to begin is Associate in nursing undefined heterogeneous cluster of molecules in answer, like a cell lysate, growth medium or serum. The molecule of interest can have a accepted and outlined property, and might be exploited throughout the affinity purification method. The method itself is thought of as Associate in nursing demurrer, with the target molecule turning into at bay on a solid or stationary part or medium. The opposite molecules within the mobile part won't become at bay as they are doing not possess this property. The stationary part will then be far away from the mixture, washed and also the target molecule free from the demurrer in an exceedingly method referred to as extraction. Presumably the foremost common use of affinity action is for the purification of recombinant proteins.