Journal of Chromatography & Separation Techniques

Journal of Chromatography & Separation Techniques
Open Access

ISSN: 2157-7064

+32 25889658

Instructions for Authors

Journal of Chromatography & Separation Techniques publishes articles bimonthly in all areas related to Analytical Chemistry. Specific topics include Advances in Chromatography, HPTLC: High performance Thin Layer Chromatography, FPLC: Fast Protein Liquid Chromatography, UHPLC: Ultra High Performance Liquid Chromatography, HPLC chromatogram analysis, GC-MS: Gas Chromatography and Mass-Spectrometry, C18 columns, Super Critical Fluid Chromatography, Preparative Chromatography, Biomedical Chromatography, Analytical Chromatography, Immuno Affinity Chromatography, Capillary Chromatography, Ion Exchange Chromatography, Gel Permeation Chromatography, Size Exclusion Chromatography, Paper Chromatography, LC-MS: Liquid Chromatography and Mass Spectrometry, Pyrolysis Gas Chromatography, Counter Current Chromatography, Agarose-gel Chromatography, Dye-ligand Chromatography, Hydrophobic Interaction Chromatography, Pseudoaffinity Chromatography, Planar Chromatography, Optical Force Chromatography, Convergence Chromatography, Micellar Electrokinetic Chromatography, MALDI-TOF Mass Spetrometry, Spectroscopic Methods, Chiral Separations, Nanofluidic and Microfluidic Separations, Ion Supression and Matrix-effects in separation, NMR Spectroscopy, Atomic Absorption Spectroscopy, HPLC-ASS, GC-ASS, LC-NMR, LC-MS-MS, EC-MS, CE-MS, GC-NMR, ICP-MS, ICP-OES, GC-IR, LC-FTIR, GC-MS, RPHPLC, HR-MS, Proteomics, Lipidomics, Protein purification, DNA fingerprinting, Extraction, Evaporation, Centrifugation, Crystallization, Distillation, Decantation, Sublimation, Magnetic Separation, Precipitation, Filtration, Adsorption, Electrophoresis, Thermogravimetric analysis, etc.

The Journal of Chromatography & Separation Techniques welcomes the submission of manuscripts that meet the general criteria of significance and scientific excellence.

As a member of Publisher International linking Association (PILA), Journal of Chromatography and Separation Techniques (of Longdom Publishing SL) adopts the Creative Commons Attribution License and Scholars Open Access publishing policies.

Submit manuscript at or send as an e-mail attachment to the Editorial office at [email protected]

A manuscript number will be e-mailed to the corresponding author within 72 hours.

Note: Authors are solely responsible for any scientific misconduct including plagiarism in their research articles; publisher is not responsible for any scientific misconduct happened in any published research article. As a publisher we follow strict scientific guidelines and EIC’s advice to retract or erratum of any article at any time if scientific misconduct or errors happen in any articles.

Publication Ethics and Malpractice Statement: 

The Journal of Chromatography & Separation Techniques abides by the ethical matters and errors and would also conduct a legal review if required. The journal ensures reprinting or advertising does not influence the decisions of editors. The Editorial Board of the Journal allows you to communicate with other publishers, journals and authors on request for connection.

Author Responsibilities:

An author is expected to present the account of work in a genuine manner along with the significance. The authors are expected to present original works and an appropriate citation should be made on citing the works of others.

An author should not include the same research in more than one manuscript for a primary publication or journal. The reported scope of work should be based on proper citation from the other influencing publications.

Any financial or personal interest that governs the findings or research in the manuscript, along with the details of financial support and its sources, should be revealed.

Longdom Publishing SL Policy Regarding the NIH Mandate:

Longdom Publishing SL will support authors by posting the published version of articles by NIH grant-holders to PubMed Central immediately after publication.

Editorial Policies and Process:

The Journal of Chromatography & Separation Techniques follows a progressive editorial policy that encourages researchers to submit the original research, reviews and editorial observations as articles, well supported by tables and graphic representation.

Article Processing Charges:

Journal of Chromatography and Separation Techniques is organized by Longdom Publishing SL, a self supporting organization and does not receive funding from any institution/government. Hence, the operation of the Journal is solely financed by the handling fees received from authors and some academic/corporate sponsors. The handling fees are required to meet maintenance of the journal. Being an Open Access Journal, Journal of Journal of Chromatography and Separation Techniques does not receive payment for subscription, as the articles are freely accessible over the internet. Authors of articles are required to pay a fair handling fee for processing their articles. However, there are no submission charges. Authors are required to make payment only after their manuscript has been accepted for publication.

The basic article processing fee or manuscript handling cost is 1819 Euro on the other hand it may vary based on the extensive editing, colored effects, complex equations, extra elongation of number of pages of the article, etc.

Submission of an Article:

In order to reduce delays, authors should adhere to the level, length and format specified at every stage of processing right from manuscript submission to each revision stage. Submitted articles should have a 300 words summary/abstract, separate from the main text. The summary should provide a brief account of the work by clearly stating the purpose of the study and the methodology adopted, highlighting major findings briefly. The text may contain a few short subheadings of no more than 40 characters each.
In order to accomplish its vision to make scientific & health care information open access and to disseminate the scientific knowledge all around the world Longdom Publishing SL made a new initiation.

Formats for Longdom Publishing SL Contributions: Longdom Publishing SL accepts the following: original articles, reviews, abstracts, addendums, announcements, article-commentaries, book reviews, rapid communications, letters to the editor, annual meeting abstracts, conference proceedings, calendars, case-reports, corrections, discussions, meeting-reports, news, obituaries, orations, product reviews, hypotheses and analyses.

Cover Letter: All submissions should be accompanied by a 500 words or less cover letter briefly stating the significance of the research, agreement of authors for publication, number of figures and tables, supporting manuscripts, and supplementary information.

Also, include current telephone and fax numbers, as well as postal and E-mail address of corresponding author to maintain communication.

Article Preparation Guidelines:

Manuscript title: The title should be limited to 25 words or less and should not contain abbreviations. The title should be a brief phrase describing the contents of the paper.

Author Information: Complete names and affiliation of all authors, including contact details of corresponding author (Telephone, Fax and E-mail address).

Abstract: The abstract should be informative and completely self-explanatory, briefly present the topic, state the scope of the experiments, indicate significant data, and point out major findings and conclusions. The abstract should summarize the manuscript content in 300 words or less. Standard nomenclature should be used and abbreviations should be avoided. The preferable format should accommodate a description of the study background, methods, results and conclusion. Following the abstract, a list of keywords (3-10) and abbreviations should be included.


Introduction: The introduction should set the tone of the paper by providing a clear statement of the study, the relevant literature on the study subject and the proposed approach or solution. The introduction should be general enough to attract a reader’s attention from a broad range of scientific disciplines.

Materials and Methods: This section should provide a complete overview of the design of the study. Detailed descriptions of materials or participants, comparisons, interventions and types of analysis should be mentioned. However, only new procedures should be described in detail; previously published procedures should be cited and important modifications of published procedures should be mentioned briefly. Capitalize trade names and include the manufacturer's name and address.

Results: The results section should provide complete details of the experiment that are required to support the conclusion of the study. The results should be written in the past tense when describing findings in the authors'experiments. Previously published findings should be written in the present tense. Results and discussion may be combined or in a separate section. Speculation and detailed interpretation of data should not be included in the results but should be put into the discussion section.

Acknowledgement: This section includes acknowledgment of people, grant details, funds etc.

Note: If an author fails to submit his/her work as per the above instructions, they are pleased to maintain clear titles namely headings, subheading.


Only published or accepted manuscripts should be included in the reference list. Meeting abstracts, conference talks, or papers that have been submitted but not yet accepted should not be cited. All personal communications should be supported by a letter from the relevant authors.

Longdom Publishing SL uses the numbered citation (citation-sequence) method. References are listed and numbered in the order that they appear in the text. In the text, citations should be indicated by the reference number in brackets. Multiple citations within a single set of brackets should be separated by commas. When there are three or more sequential citations, they should be given as a range. Example: "... now enable biologists to simultaneously monitor the expression of thousands of genes in a single experiment [1,5-7,28]". Make sure the parts of the manuscript are in the correct order for the relevant journal before ordering the citations. Figure captions and tables should be at the end of the manuscript.

Authors are requested to provide at least one online link for each reference as following (preferably PubMed).

Because all references will be linked electronically as much as possible to the papers they cite, proper formatting of the references is crucial. Please use the following style for the reference list:


Published Papers:

  1. Maher HM. Stacking As Sample On-Line Pre-concentration Technique in Microemulsion Electrokinetic Chromatography. J Chromatogr Sep Tech. 2015;6(3):e130.
  2. Bava M, Arnoldi S, Dell’Acqua L, Fontana S, Forgia FL, Mustich G et al. Quali-Quantitative Analysis by Lc/Dad and Gpc of the Polyphenols of “Uva Di Troia Canosina” Grape Seeds for the Development of an Industrial Nutraceutical Product. J Chromatogr Sep Tech. 2015;6(3):266.
  3. Mottaleb MA, Bellamy MK, Mottaleb MA, Islam MR. Use of LCMS and GC-MS Methods to Measure Emerging Contaminants Pharmaceutical and Personal Care Products (PPCPs) in Fish. J Chromatogr Sep Tech. 2015;6(3):267.

Note: Please list the first six authors and then add "et al." if there are additional authors.

Electronic Journal Articles Entrez Programming Utilities:



  1. Baggot JD. Principles of drug disposition in domestic animals: The basis of Veterinary Clinical Pharmacology. (1stedn), W.B. Saunders Company, Philadelphia, London, Toranto. 1999.
  2. Zhang Z. Bioinformatics tools for differential analysis of proteomic expression profiling data from clinical samples. Taylor & Francis CRC Press. 2006.


  1. Hofmann T. The Cluster-Abstraction Model: unsupervised learning of topic hierarchies from text data. Proceedings of the International Joint Conference on Artificial Intelligence. 1999.


These should be used at a minimum and designed as simple as possible. We strongly encourage authors to submit tables as .doc format. Tables are to be typed double-spaced throughout, including headings and footnotes. Each table should be on a separate page, numbered consecutively in Arabic numerals and supplied with a heading and a legend. Tables should be self-explanatory without reference to the text. Preferably, the details of the methods used in the experiments should be described in the legend instead of in the text. The same data should not be presented in both table and graph form or repeated in the text. Cells can be copied from an Excel spreadsheet and pasted into a word document, but Excel files should not be embedded as objects.

Note: If the submission is in PDF format, the author is requested to retain the same in .doc format in order to aid in completion of process successfully.


The preferred file formats for photographic images are .doc, TIFF and JPEG. If you have created images with separate components on different layers, please send us the Photoshop files.

All images must be at or above intended display size, with the following image resolutions: Line Art 800 dpi, Combination (Line Art + Halftone) 600 dpi, Halftone 300 dpi. See the Image quality specifications chart for details. Image files also must be cropped as close to the actual image as possible.

Use Arabic numerals to designate figures and upper case letters for their parts (Figure 1). Begin each legend with a title and include sufficient description so that the figure is understandable without reading the text of the manuscript. Information given in legends should not be repeated in the text.

Figure legends: These should be typed in numerical order on a separate sheet

Tables and Equations as Graphics:

If equations cannot be encoded in MathML, submit them in TIFF or EPS format as discrete files (i.e., a file containing only the data for one equation). Only when tables cannot be encoded as XML/SGML can they be submitted as graphics. If this method is used, it is critical that the font size in all equations and tables is consistent and legible throughout all submissions.

  • Suggested Equation Extraction Method
  • Table Specifications
  • Equation Specifications

Supplementary Information:

Discrete items of the Supplementary Information (for example, figures, tables) referred to at an appropriate point in the main text of the paper.

Summary diagram/figure included as part of the Supplementary Information (optional).

All Supplementary Information is supplied as a single PDF file, where possible. File size within the permitted limits for Supplementary Information. Images should be a maximum size of 640 x 480 pixels (9 x 6.8 inches at 72 pixels per inch).

Proofs and Reprints: 

Electronic proofs will be sent as an e-mail attachment to the corresponding author as a PDF file. Page proofs are considered to be the final version of the manuscript. With the exception of typographical or minor clerical errors, no changes will be made in the manuscript at the proof stage. Authors will have free electronic access to the full text (HTML, PDF and XML) of the article. Authors can freely download the PDF file from which they can print unlimited copies of their articles.


Submission of a manuscript implies that the work described has not been published before (except in the form of an abstract or as part of a published lecture, or thesis) and that it is not under consideration for publication elsewhere.

All works published by Longdom Publishing SL are under the terms of the Creative Commons Attribution License. This permits anyone to copy, distribute, transmit and adapt the work provided the original work and source is appropriately cited.


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