Mass Spectrometry & Purification Techniques

Mass Spectrometry & Purification Techniques
Open Access

ISSN: 2469-9861

Protein Purification

Protein purification is a series of processes to isolate protein from a complex mixture (cells, tissues or whole organisms) to one or a few proteins. Protein purification mainly depends on the structure and interactions of the protein of interest however thousands of proteins of each displaying unique characteristics. Protein purification is essential for a host of biochemical applications. It is the most important to develop a strategy for purification that delivers the correct yield, purity and activity needed for downstream applications. Primary steps for protein purification are extraction, precipitation/differential solubilization and ultracentrifugation.

Protein purification strategy consist one or more chromatographic steps like size exclusion chromatography, separation based on charge or hydrophobicity (hydrophobic interaction chromatography, ion exchange chromatography, Free-flow Electrophoresis), affinity chromatography(Metal binding, immunoaffinity chromatography, Purification of a tagged protein), HPLC methods.

Related journals of Protein Purification
Journal of Bioanalysis & Biomedicine, Journal of Analytical & Bioanalytical Techniques, Protein Expression and Purification, Recombinant protein expression and purification.

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