Journal of Nutrition & Food Sciences
Open Access
ISSN: 2155-9600
ISSN: 2155-9600
Zhaowei Zhang, Peiwu Li and Qi Zhang
Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, China
Ministry of Agriculture, China
Posters & Accepted Abstracts: J Nutr Food Sci
Multiplexed mycotoxins with strong carcinogenesis and toxicity are fatal threats in food safety, and require highly sensitive and high-throughput detections greatly. Rapid immunoassay and confirming detection methods play a critical role on two sides of one coin. In the rapid immunoassay, a series of high specific and high affinity monoclonal antibody, recombinant antibody, and nanobody against aflatoxin B1 (AFB1), ochratoxin A (OTA), and zearalenone (ZEA), etc, were developed as the key recognition reagents. Based on these specific antibodies, simultaneous detection for multiplexed mycotoxins was studied by using the Au (or Europium)-based lateral flow strip and non-fouling antigen microarray. The limit of detection was lowered down to pg/mL level (as 0.3 pg/mL), depending on mycotoxins in food samples. On the other hand, simultaneous confirming detection method based on HPLC-MS/MS was investigated. Either multiplexed immunoaffinity column or solid phase extraction column was used in the sample extraction. The internal standard allowed precise determination of mycotoxins regardless of matrices. Multiplexed mycotoxins (AFB1, B2, G1, G2, OTA, ZEA, etc.) were successfully identified by using a multi-immunoaffinity column in a single run. Furthermore, a promising proposal was suggested to achieve the rapid, sensitive, ultra high-throughput detection of 96-384 contaminants in food and feed, including biotoxins, pesticides, veterinary drugs, etc., based on immunochemiluminescence biosensors using Hadamard transformation imaging (iHT).
Email: zwzhang@whu.edu.cn