Molecular characterization of enterohemorrhagic Escherichia coli O157:H7 in dairy foods and environments
Journal of Nutrition & Food Sciences

Journal of Nutrition & Food Sciences
Open Access

ISSN: 2155-9600

Molecular characterization of enterohemorrhagic Escherichia coli O157:H7 in dairy foods and environments

10th World Congress on Nutrition & Food Sciences

May 29-31, 2017 Osaka, Japan

Sang Don Ryu, Mi Ri Park and Sangnam Oh

Chonbuk National University, Korea

Posters & Accepted Abstracts: J Nutr Food Sci

Abstract :

The entry route of foodborne pathogens including EHEC O157:H7 from the dairy farm environment into raw milk and dairy foods may come from several different vectors, and influence on the human health. Here, we employed HT-29 intestine epithelial cells (IECs; in vitro) and C. elegans (in vivo) as host model systems and compared the specific host responses during EHEC O157:H7 infections using whole transcriptome analysis. To elucidate the immune pathways stimulated by EHEC O157, we employed quantitative real time-polymerase chain reaction (qRT-PCR), transgenic worms, and RNAi. After EHEC O157:H7 infections, the regulation of gene encoding detoxification process, stress response, immune response and oxidative stress-associated proteins were immediately remodeled. Among them, we showed that the genes encoding cytochrome P450 (CYP450) family were dramatically induced by >10- fold during infection of EHEC O157:H7 is common in both host models. Importantly, C. elegans mutants lacking CYP450 genes were highly susceptible to EHEC O157:H7 infections comparing to wild-type N2. Consistent with susceptibility tests, qRT-PCR results showed that CYP450 loss of function mutations significantly affected the transcriptional induction of antimicrobial peptide genes such as clec-60. Taken in their totality our results provide critical insights for host strategy to exclude EHEC O157:H7 pathogenesis in gastrointestinal (GI) tracts through cytochrome P450 family.

Biography :

Sang Don Ryu has great experience on detection and characterization of foodborne pathogens in food microbiology research. In recent, he focused on the prevalence and molecular pathway of various foodborne pathogens including Listeria monocytogenes, Staphylococcus aureus, Camphylobacter jejuni, Salmonella typhumurium, and pathogenic E. coli isolated from dairy foods and environments. Especially, his outstanding results may help monitor and control the spread of bacterial species through the farm environment, which is a natural reservoir for these organisms.

Email: [email protected]