Journal of Nutrition & Food Sciences
Open Access

Modified University of Wisconsin solution with melatonin and its efficacy on kidney preservation time

6^th International Conference and Exhibition on Nutrition

September 14-16, 2016 San Antonio, USA

S Arbak, A Coskun, C Yegen, O Gunal, W Attaallah, I Seckin, B Cetiner, Z Yildirim, Y Erdemgil, M A Elmas, S Demirci, O Can, M Serteser, A Ozp�?±nar, G Basdemir, A Belce and I Unsal

Acibadem University, Turkey
Marmara University, Turkey
Istanbul University, Turkey
Memorial Hospital, Turkey
Bezmialem Vakif University, Turkey

Posters & Accepted Abstracts: J Nutr Food Sci

Abstract :

In order to provide long lasting time period for organ preservation, donor organs are kept in special preservation solutions, as University of Wisconsin (UW) is the most preferred solution. The aim of this study is to extend the preservation time and also provide more effective protection. In order to provide better preservation, the ingredients of the UW solution were changed (Modified UW), as well as melatonin was included (Modified UW+M) in the preservation medium. Time related morphological changes of rat kidneys in each group were comparatively investigated within this study. Totally perfused kidneys were placed in UW, Modified UW and Modified UW+M solutions and kept during 2, 10, 24 and 72 h at 4 oC. Kidney tissue samples were taken at all given time intervals and these samples were prepared for light and transmission electron microscopy. Histopathological scoring based on renal injury, tubular and glomerular degeneration, inflammatory cell infiltration and vasocongestion was performed. Liquid samples, taken at 2, 10, 24 and 72 h at 4 oC from the storage media were investigated for lactate dehydrogenase (LDH) activity. Comparative findings at light and transmission electron microscopical levels and also LDH results revealed that preservation in Mod UW+M solution was statistically much more prominent in all time intervals, significantly at 72nd hour of preservation. In all groups, LDH levels were consistent with morphological results. We could conclude that Mod UW+M were the most effective solution among the experimental groups especially suitable for preservation up to 72 hours.

Biography :

Email: arbaks@yahoo.com