Journal of Nutrition & Food Sciences
Open Access
ISSN: 2155-9600
ISSN: 2155-9600
Prof. Jeswanth Sathyanesa
Government Stanley Medical College and Hospital in Chennai, Tamil Nadu, India
Scientific Tracks Abstracts: J Nutr Food Sci
Dysbiosis of the gut microbiota is a hallmark of inflammatory bowel disease (IBD). Harnessing beneficial microbes (probiotics) and their metabolic products (postbiotics) offers a promising adjunct to conventional therapies. Naturally fermented rice is a rich source of lactic acid bacteria and bioactive metabolites that may support gut barrier function and modulate mucosal immunity. Methods: Rice fermentations were incubated at 37 °C for 72 h. Probiotic candidates were isolated via anaerobic culture, identified by 16S rRNA sequencing, and screened for acid/bile tolerance, adhesion to Caco-2 cells, and antimicrobial activity against Escherichia coli and Clostridioides difficile. Cell-free supernatants (postbiotic fractions) were chemically profiled by HPLC–MS for short-chain fatty acids (SCFAs) and phenolic compounds. Their effects on intestinal epithelial monolayer integrity (TEER assay) and LPS-induced cytokine release (IL-8, TNF-α ELISA) were evaluated in Caco-2 and THP-1 macrophage co-culture models. Results: Two Lactobacillus plantarum strains (LP-RF1, LP-RF2) and one Pediococcus pentosaceus (PPRF3) met probiotic criteria, exhibiting >80 % survival in simulated gastric conditions and strong adhesion (adhesion index > 100). Postbiotic fractions contained high concentrations of acetate, propionate, and butyrate (total SCFAs 250 mM) plus gallic and ferulic acids. Treatment with LP-RF1 and LP-RF2, or their postbiotics (5 % v/v), significantly (p < 0.01) increased TEER by 30 % and reduced LPS-induced IL-8 and TNF-α secretion by 45 % and 50 %, respectively.
Professor Jeswanth Sathyanesan is a faculty member at the Institute of Surgical Gastroenterology and Liver Transplantation, located within the Centre for GI Bleed, Division of Hepatobiliary Pancreatic Diseases at the Government Stanley Medical College and Hospital in Chennai, Tamil Nadu, India. His areas of expertise and research interests include surgical gastroenterology, liver transplantation, and hepatobiliary and pancreatic diseases.