Journal of Nutrition & Food Sciences
Open Access
ISSN: 2155-9600
+32 25889658
ISSN: 2155-9600
+32 25889658
Akin demet, Farah adriana, Demircan gunnur, Beyhan ozdas sule
Bahcesehir University, Turkey
Federal University of Rio de Janeiro, Brazil
Istanbul Bilim University, Turkey
Posters & Accepted Abstracts: J Nutr Food Sci
Coffee is particularly rich in bound phenolic acids, such as caffeic acid, ferulic acid, and p-coumaric acid. Over the past years, several studies have shown that Ferulic acid acts as a potent antioxidant by scavenging free radicals. Autophagy has recently been considered as a protective mechanism during the development of atherosclerosis because of its ability to stabilize plaques through the processing of oxidatively modified proteins. Our objective was to study the effect of ferulic acid on autophagy in cultured human umbilical vein endothelial cells (HUVECs) undergoing oxidative stress. LC3B and Beclin1 protein expression levels were measured by western blott and immunocytochemistry in oxidative stress conditions induced by administration of 200 microM hydrogen peroxide (H2O2) for 1 hour. After H2O2 administration cells were washed with DMEM and incubated 24 hours with 100 microM ferulic acid (FA). Ascorbic acid, a known antioxidant, has been used as positive control. Ferulic acid decreased the ratio of LC3II/LC3I and Beclin-1 protein levels in oxidative stress conditions. We found similar results in immunocytochemistry studies. Our preliminary data suggest the novel cardioprotective properties of ferulic acid mediated by targeting the autophagic pathway. Ferulic acid may have the potential for use as an autophagic-related antioxidant for prevention and treatment of oxidative stress.
Email: demet.akin@med.bau.edu.tr