Journal of Nutrition & Food Sciences
Open Access
ISSN: 2155-9600
ISSN: 2155-9600
Kazunobu Baba and Tadaaki Miyazaki
Hokkaido University, Japan
Posters & Accepted Abstracts: J Nutr Food Sci
Lactobacillus helveticus SBT2171 (LH2171: provided by Megmilk Snow Brand Co., Ltd.) is a lactic acid bacterium with high protease activity and used in starter cultures in the manufacturing of cheese. We have previously revealed that consumption of cheese using LH2171 alleviated symptoms of dextran sodium sulfate (DSS)-induced colitis in mice. In this study, we investigated the effect of LH2171 on the proliferation of MC38, a mouse colon carcinoma cell line and HT-29, a human colorectal adenocarcinoma cell line. LH2171 inhibited the proliferation of both MC38 cells and HT-29 cells. As a result of assay for the phosphorylation of cell growth regulators in MC38 cells, LH2171 inhibited the activation of JNK signaling pathway. In addition, LH2171 induced the expression of JNK-inactivating phosphatase, MKP1 at a transcriptional level and MKP1 siRNA attenuated the effect of LH2171 to inhibit cell proliferation. Furthermore, this regulation by LH2171 occurred by LH2171-induced TGF-1 autocrine manner. Subsequently, LH2171 released TGF-1 at a transcriptional level through the activation of extracellular signal-regulated kinase (ERK). These results indicate that the inhibitory effect of LH2171 on the proliferation of MC38 cells depends on the activation of ERK-TGF-1 axis and concomitant induction of MKP1 to inhibit JNK activity and cell proliferation.