Journal of Nutrition & Food Sciences
Open Access
ISSN: 2155-9600
ISSN: 2155-9600
Farah Ashfaq
Lahore College for Women University, Pakistan
Posters & Accepted Abstracts: J Nutr Food Sci
Obesity in adolescents and young adults has increased significantly in recent years resulting in the development of chronic diseases. Obesity induces adipocyte dysfunction, secretion of adipokines and activation of macrophages leading to inflammatory cytokine production. The aim of the present study was to investigate the adiponectin gene expression in young subjects of different BMI groups. Study subjects included 300 overweight, obese males and females with an age ranging from 17�??30 years. 100 comparable control subjects with normal BMI were included. The data was stratified into normal-weight, overweight, obese I and obese II groups. Anthropometric parameters including age, BMI, waist circumference, WHR, systolic and diastolic blood pressure were assessed. The metabolic and inflammatory parameters including glucose, insulin, lipid profile, leptin, adiponectin, resistin, C-reactive protein and interleukin-6 in serum were measured by chemistry analyzer and ELISA. RNA extraction was done by TRIZOL method and cDNA synthesis was done by using cDNA synthesis kit. The expression of target gene was compared with GAPDH on real time PCR using gene specific primers. Serum levels of insulin, leptin, resistin, CRP, IL-6 were significantly higher in overweight and obese subjects as compared to control subjects (p<0.01). Adiponectin was significantly low in obese groups (p<0.01). In correlation analysis, adiponectin showed a significant inverse relationship with BMI, WC (r =-.262, p<.008), (r=-.310, p<.002) p<0.01 and with WHR (r=-.199, p=.046) p<0.05, respectively. Adiponectin was significantly correlated with fasting insulin (r=-.282, p=.004) p<0.01 in obese group. The expression of adiponectin gene was significantly influenced by obesity.
E-mail: farah_ashfaq@yahoo.com