Panjab University, India
Posters-Accepted Abstracts: J Nutr Food Sci
Aconitum heterophyllum Wall. is consumed for its promising medicinal properties in several parts of the world. Present study consists of hydro distillation, antioxidant potential and in-silico antiapoptotic study of A. heterophyllum oil. Antioxidant activities were evaluated by in-vitro assays i.e., 2, 2-diphenyl-1-picrylhydrazyl (DPPH), Cupric Reducing Antioxidant Capacity (CUPRAC) and Superoxide inhibitor. It was found that the anti-oxidative effect of A. heterophyllum oil was dose dependent upto 200 Ã?Â¼g/ml. For studying the apoptotic nature of the volatile constituents, in silico studies were carried out using BCL-2 anti-apoptotic receptors (BCL-2, BCL-XL and MCL-1). To understand the cascade of mechanisms leading to apoptosis, NF- Ã?ÂºB was also considered. Docking score of various constituents with specific receptors were compared and it was found that Ã?Â²-fenchol binds best with NF-Ã?ÂºB with the dock score of -4.36 kcal/ mol as compared to the selective inhibitors parthenolide (dock score was -3.04 kcal/mol). However, the constituents show favorable binding affinity for the receptors in the case of BCL- 2 receptor, Ã?Â±-longipinene has a dock score of -4.26 kcal/mol as compared to that of standard inhibitor ABT 263 (-4.67 kcal/ mol); Another constituent i.e., neryl acetate shows a dock score of -4.05 kcal/mol with BCL-XL as compared to stand inhibitor ABT 737 (-9.47 kcal/mol).
Yashika is a researcher at University institute of Pharmaceutical Sciences, Panjab University, India
Email: [email protected]