Journal of Food: Microbiology, Safety & Hygiene
Open Access
ISSN: 2476-2059
+44 1478 350008
ISSN: 2476-2059
+44 1478 350008
Alexandr E Urusov, Miliausha K Gubaidullina, Anatoly V Zherdev and Boris B Dzantiev
Research Center of Biotechnology RAS, Russia
Posters & Accepted Abstracts: J Food Microbiol Saf Hyg
Mycotoxins are one of the priority classes of agricultural products contaminants. They pose a significant danger to human and animal health. For productive monitoring of these toxic contaminants new formats of Immunochromatographic (ICA) and Immunoenzyme Assays (IEA) have been developed. For highly sensitive detection in indirect labeling ICA, the combination of non-modified specific antibodies and colloidal gold nanoparticles-labeled anti-species antibodies (anti-IgGGNP) is proposed. This approach allows decreasing limits of detection in 10-20 times (for example, for zearalenone to 350 pg/ ml). The assay duration varies from 10 to 20 min. For IEA, the use of immunosorbents based on magnetic nanoparticles is proposed. The given approach allows concentrating the analyte and performing immunochemical interactions in homogeneous, diffusion-independent mode, thereby reducing the detection limit and the duration of the analysis. The total IEA duration is reduced by 3-4 times - up to 20-30 min (with 5-min specific immune interaction). The limit of detection is decreased on an order of magnitude as compared with common heterogeneous microplate IEA, accounting for example, 2 pg/ml for aflatoxin B1. The developed assays for aflatoxin B1, ochratoxin A, zearalenone and T2-toxin ensure 90% or higher revealing the analyte in cereals, thus indicating prospects of the proposed approaches. Recent Publications: 1. Petrakova A V, Urusov A E, Gubaydullina M K, Bartosh A V, Zherdev A V and Dzantiev B B (2017) External antibodies as the simplest tool for sensitive immunochromatographic tests. Talanta 175:77-81. 2. Urusov A E, Petrakova A V, Zherdev A V and Dzantiev B B (2016) Multistage in one touch design with a universal labelling conjugate for high-sensitive lateral flow immunoassays. Biosensors and Bioelectronics, 86: 575-579. 3. Urusov A E, Zherdev A V, Petrakova A V, Sadykhov E G, Koroleva O V and Dzantiev B B (2015) Rapid multiple immunoenzyme assay of mycotoxins. Toxins 7(2):238-254. 4. Urusov A E, Zherdev A V and Dzantiev B B (2014) Use of gold nanoparticle-labeled secondary antibodies to improve the sensitivity of an immunochromatographic assay for aflatoxin B1. Microchimica Acta 181(15-16):1939-1946. 5. Urusov A E, Petrakova A V, Vozniak M V, Zherdev A V and Dzantiev B B (2014) Rapid immunoenzyme assay of aflatoxin B1 using magnetic nanoparticles. Sensors 14:21843-21857.
Alexandr E Urusov obtained his Doctor degree in Chemistry in A.N. Bach Institute of Biochemistry in 2012. Now he is Researcher in Research Center of Biotechnology of the Russian Academy of Sciences. His research interests molecular immunology, immunology, methods and apparatus in immunology, biotechnology, immunoenzyme analysis, medicine, health care and medical biochemistry.
Email:urusov.alexandr@gmail.com