Detection of virulence and antibiotic resistance genes in Aeromon | 43455
Journal of Nutrition & Food Sciences

Journal of Nutrition & Food Sciences
Open Access

ISSN: 2155-9600

Detection of virulence and antibiotic resistance genes in Aeromonas Sp in Sub-Himalayan West Bengal, India

21st International Conference on Food & Nutrition

July 25-26, 2018 | Vancouver, Canada

Dipanwita Saha

University of North Bengal, India

Scientific Tracks Abstracts: J Nutr Food Sci

Abstract :

Aeromonas spp., abundantly found in aquaculture systems, are implicated as pathogens associated with fish and human diseases. Owing to their complexity with respect to virulence, increasing host range and rapidly evolving antibiotic resistance mechanisms, it becomes important to investigate the genetic basis and dissemination of associated genes. Biochemical characterization known as aerokey II, was used to identify 34 strains of Aeromonas isolates in aquaculture bodies of sub-Himalayan West Bengal. Molecular detection was carried on the basis of self designed 16S rRNA gene primers specific to Aeromonas. Isolates were tested phenotypically for the presence of haemolysin, protease and DNase, reported as virulence factors in motile Aeromonas. Corresponding genetic determinants aer/haem (haemolytic toxin), aspA (serine protease), ascV (Type3 secretion system) and flaA (polar flagella) were investigated. All isolates were found to be beta haemolytic and proteolytic whereas 73.52 % were positive for DNase. While 44.11% harboured aer/haem gene, 23.54% showed ascV gene. aspA was detected in 11.76% whereas 41.17 % exhibited the occurrence of flaA gene. In vivo pathogenicity of 6 virulent strains was tested in fish (Anabas sp.). Severe lesions and mortality was observed in 60 % and 18.2% of the fishes respectively within 7 days. A MAR index value >0.2 was detected among 14.7% of the isolates subjected to antibiotic sensitivity testing against 19 antibiotics. Presence of class I integrons was also detected in 26.5% of the strains. These findings provide greater insights of the prevalence and infectious nature of Aeromonas posing a threat to public health.

Biography :

Dipanwita Saha has completed her graduation in Biochemistry in 1991 from Calcutta University and doctoral studies from Centre for Life Sciences, University of North Bengal in 2000. She is an Assistant Professor (stage III) in Biotechnology in University of North Bengal. She has received best presentation award in several occassions and published more than 34 papers in reputed journals. She has visited USA, France, Thailand, Egypt and UK for academic purposes.