Journal of Nutrition & Food Sciences
Open Access
ISSN: 2155-9600
ISSN: 2155-9600
Umesh Kumar Shandilya, Rajeev Kapila, S. Kapila and V. K. Kansal
AcceptedAbstracts: J Nutr Food Sci
The present study was carried out to evalaute the effect of feeding probiotic Dahi on whey proteins (WP) allergenicity in mice. Probiotic Dahi were prepared by co-culturing in buffalo milk (3% fat) Dahi bacteria (Lactococcus lactis ssp. cremoris NCDC- 86 and Lactococcus lactis ssp. lactis biovar diacetylactis NCDC- 60) along with selected strain of Lactobacillus acidophilus LaVK2 (La-Dahi) or combined L. acidophilus and Bifidobacterium bifidum BbVK3 (LaBb-Dahi). Four groups of mice (8 each) were fed with the supplements (5 g/day) of buffalo milk (3% fat), Dahi, La-Dahi and LaBb-Dahi from 7 days before sensitization with WP (20μg/200μl PBS), respectively, in addition to milk protein-free basal diet, and a fifth group that received no supplements served as control. Feeding of probiotic Dahi suppressedthe elevation of whey proteins-specific IgE and IgG response in serum of WP-sensitized mice. In addition,sIgA levels were significantly (p<0.001) increased in intestinal fluid collected frommice fed with La-Dahi or LaBb-Dahi.Production of T helper (Th)-1 cell-specific cytokines, i.e. interferon-γ (IFN-γ), interleukin (IL)-12, and IL-10 increased, while Th2-specific cytokines, i.e. IL-4 decreased in the supernatant of cultured splenocytes collected from mice fed with probiotic Dahi compared to the other groups. Moreover,the splenic mRNA levels of IFN-γ, interleukin-10 were found to be significantly increased, while that of IL-4 decreased significantly in La-Dahi or LaBb-Dahi groups, as compared to control groups. Taken together, these results indicate that administration of La-Dahi and LaBb-Dahi might be useful for prevention of milk proteins allergy.