Journal of Nutrition & Food Sciences
Open Access
ISSN: 2155-9600
ISSN: 2155-9600
RCMI Center for Health Disparities Research, Jackson State University, 1400 Lynch Street, Box 18750,, United States of America
Research Article
Garlic Extract Triggers Cytotoxicity, DNA Damage, and Cell Cycle Arrest in Human Leukemic Cells via Oxidative Stress
Author(s): Sylvianne Njiki*, Keara Johnson, Jennifer N. Sims, Ariane M. Chitoh, Clement G. Yedjou*, Felicite K. Noubissi, Barbara Graham and Paul B. Tchounwou*
Garlic (Allium sativum) is one of the oldest medicinal plants that has received great attention throughout ancient and modern history. It has been used for the prevention and treatment of a wide variety of ailments, including cancer, cardiovascular disease, and hepatic and microbial infections. Although garlic shows potential in the prevention and treatment of cancer and other diseases, little is known about its therapeutic mechanisms of action. Here, we hypothesized that Garlic Extract (GE) induces cytotoxicity, DNA damage, and cell cycle arrest in Human Leukemia (HL-60) cells through oxidative stress. To test our hypothesis, HL-60 cells were treated with different concentrations (2 mg/mL, 4 mg/mL, and 6 mg/mL) of GE for 24 hours. Cell viability and cell morphology were analyzed by MTT, trypan blue, and Acridine Orange and Propidium Iodide (AO/PI) assays, respectively. The e.. View More»
DOI:
10.35248/2155-9600.25.15.67