Journal of Chromatography & Separation Techniques
Open Access
ISSN: 2157-7064
ISSN: 2157-7064
Zvereva I
Spain
Research Article
Detection of Growth Hormone Releasing Peptides in Serum by a
Competitive Receptor Binding Assay
Author(s): Ferro P, Krotov G, Zvereva I, Perez- Mana, Mateus JA and Segura J
Ferro P, Krotov G, Zvereva I, Perez- Mana, Mateus JA and Segura J
The use of small peptides for medical purposes has gained great importance in the last decade. Among these molecules are the growth hormone releasing peptides (GHRPs). These substances are highly sought by cheating athletes because of their ergogenic effects. Thus, GHRPs are listed in the World Antidoping Agency Prohibited List and several methods have been developed for their detection in urine samples. Serum is another useful matrix frequently used for anti-doping control. An experimental adaptation of a competitive methodology for GHRPs wide-group detection, previously developed for urine, is reported here using a serum matrix instead. The modifications have been mainly addressed the need to remove endogenous ghrelin (always present in serum) to avoid interference in the competitive binding assay. The modified test was first evaluated using serum spiked with different pure GHRPs (i.. View More»
DOI:
10.4172/2157-7064.1000351