Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
ISSN: 0974-276X
Yurong Lai
Department of Pharmacokinetics, Dynamics and Metabolism, MS 8220-2475,
Pfizer Global Research and Development, Pfizer Inc., Groton, CT 06340
Tanzania
Review Article
Membrane Protein Quantification by Peptide-Based Mass Spectrometry Approaches: Studies on the Organic Anion-Transporting Polypeptide Family
Author(s): Larissa M Balogh, Emi Kimoto, Jonathan Chupka, Hui Zhang and Yurong LaiLarissa M Balogh, Emi Kimoto, Jonathan Chupka, Hui Zhang and Yurong Lai
The reliable quantification of drug transport proteins is a key element that is necessary to establish relative scaling factors for human pharmacokinetic predictions and to account for pharmacokinetic variations across populations. Herein, we report a detailed evaluation of targeted mass spectrometry-based quantification methods from the perspective of sample preparation for three major organic anion-transporting polypeptide (OATP) membrane proteins. We also demonstrate the first implementation of stable isotope labeling by amino acids in cell culture (SILAC) into the OATP quantification scheme, which hinges on label incorporation at the protein level to allow for a substantially accelerated sample preparation process with precise results. These methods will enable comparable quantifications to provide individual scaling factors as well as address potential inter-individual variabilit.. View More»
DOI:
10.4172/jpb.1000285