Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
+44 1223 790975
ISSN: 0974-276X
+44 1223 790975
Yuko Nobe
Japan
Research Article
Friend of Prmt1, FOP is a Novel Component of the Nuclear SMN Complex Isolated Using Biotin Affinity Purification
Author(s): Keiichi Izumikawa, Hideaki Ishikawa, Harunori Yoshikawa, Goro Terukina, Naoki Miyazawa, Hiroshi Nakayama, Yuko Nobe, Masato Taoka, Yoshio Yamauchi, Sjaak Philipsen, Toshiaki Isobe and Nobuhiro TakahashiKeiichi Izumikawa, Hideaki Ishikawa, Harunori Yoshikawa, Goro Terukina, Naoki Miyazawa, Hiroshi Nakayama, Yuko Nobe, Masato Taoka, Yoshio Yamauchi, Sjaak Philipsen, Toshiaki Isobe and Nobuhiro Takahashi
SMN (survival motor neuron protein) complexes are essential for the biogenesis of uridine-rich small nuclear ribonucleoproteins (UsnRNPs). During the biogenesis, the SMN complexes bound to UsnRNPs are transported from the cytoplasm to the nucleus, and moved to Cajal body (bodies)/Gems (Cajal/Gems) where the SMN complexes- UsnRNPs are subjected to additional chemical modifications and dissociated to the SMN complexes and the mature UsnRNPs. Although the mature UsnRNPs are assembled into spliceosome with newly transcribed pre-mRNA in the perichromatin fibrils at the chromatin, the role of the dissociated nuclear SMN complexes remains undetermined. In this study, we identified Friend of Prmt1 (FOP; chromatin target of Prmt1, CHTOP; C1orf77) as a novel component of the nuclear SMN complexes by the biotin affinity purification, coupled with the mass spectrometry-based protein identificatio.. View More»
DOI:
10.4172/jpb.S7-002