Journal of Proteomics & Bioinformatics

Journal of Proteomics & Bioinformatics
Open Access

ISSN: 0974-276X

+44 1223 790975

Young-Mi Go

Young-Mi Go


  • Research Article
    Protein Cysteines Map to Functional Networks According to Steady-state Level of Oxidation
    Author(s): Young-Mi Go, Duc M. Duong, Junmin Peng and Dean P. JonesYoung-Mi Go, Duc M. Duong, Junmin Peng and Dean P. Jones

    The cysteine (Cys) proteome serves critical roles in protein structure, function and regulation, and includes key targets in oxidative mechanisms of disease. Thioredoxins maintain Cys residues in thiol forms, and previous research shows that the redox potential of thioredoxin in mitochondria and nuclei is more reduced than cytoplasm, suggesting that proteins in these compartments may have different steady-state oxidation. This study measured fractional oxidation of 641 peptidyl Cys residues from 333 proteins in HT29 cells by mass spectrometry. Average oxidation of cytoplasmic, nuclear and mitochondrial proteins was similar (15.8, 15.5, 14%, respectively). Pathway analysis showed that more reduced cytoplasmic Cys were in proteins associated with the cytoskeleton, more reduced nuclear Cys with Ran signaling and RNA post-transcriptional modification, and more reduced mitochondrial Cys wi.. View More»
    DOI: 10.4172/jpb.1000190

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